pUR-sap1+
(Plasmid
#15984)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 15984 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUR18
- Backbone size w/o insert (bp) 5500
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Vector typeYeast Expression
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Selectable markersURA4 (S. pombe equivalent to URA3)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSap1
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SpeciesS. pombe (fission yeast)
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Entrez Genesap1 (a.k.a. SPCC1672.02c)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A clone isolated from pURSP1 S. pombe genomic library available from Tony Carr. S. pombe elements: ars, ura4+, sap1+.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUR-sap1+ was a gift from Eishi Noguchi (Addgene plasmid # 15984 ; http://n2t.net/addgene:15984 ; RRID:Addgene_15984) -
For your References section:
Sap1 promotes the association of the replication fork protection complex with chromatin and is involved in the replication checkpoint in Schizosaccharomyces pombe. Noguchi C, Noguchi E. Genetics. 2007 Feb . 175(2):553-66. 10.1534/genetics.106.065334 PubMed 17151242