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Addgene

pUR-sap1+
(Plasmid #15984)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15984 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pUR18
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Yeast Expression
  • Selectable markers
    URA4 (S. pombe equivalent to URA3)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sap1
  • Species
    S. pombe (fission yeast)
  • Entrez Gene
    sap1 (a.k.a. SPCC1672.02c)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A clone isolated from pURSP1 S. pombe genomic library available from Tony Carr. S. pombe elements: ars, ura4+, sap1+.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUR-sap1+ was a gift from Eishi Noguchi (Addgene plasmid # 15984 ; http://n2t.net/addgene:15984 ; RRID:Addgene_15984)
  • For your References section:

    Sap1 promotes the association of the replication fork protection complex with chromatin and is involved in the replication checkpoint in Schizosaccharomyces pombe. Noguchi C, Noguchi E. Genetics. 2007 Feb . 175(2):553-66. 10.1534/genetics.106.065334 PubMed 17151242