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pCFJ1820 - pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
(Plasmid #159838)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159838 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST R4-R3
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
  • Species
    C. elegans (nematode)
  • Mutation
    Not applicable

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See www.wormbuilder.org for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCFJ1820 - pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm] was a gift from Christian Froekjaer-Jensen (Addgene plasmid # 159838 ; http://n2t.net/addgene:159838 ; RRID:Addgene_159838)

  • For your References section:

    Engineering rules that minimize germline silencing of transgenes in simple extrachromosomal arrays in C. elegans. Aljohani MD, El Mouridi S, Priyadarshini M, Vargas-Velazquez AM, Frokjaer-Jensen C. Nat Commun. 2020 Dec 9;11(1):6300. doi: 10.1038/s41467-020-19898-0. 10.1038/s41467-020-19898-0 PubMed 33298957
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