pCFJ1820 - pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
(Plasmid
#159838)
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PurposeThree-fragment Gateway compatible pDEST vector to clone transgene downstream of smu-1 for germline expression from arrays
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 159838 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDEST R4-R3
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
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SpeciesC. elegans (nematode)
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MutationNot applicable
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See www.wormbuilder.org for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCFJ1820 - pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm] was a gift from Christian Froekjaer-Jensen (Addgene plasmid # 159838 ; http://n2t.net/addgene:159838 ; RRID:Addgene_159838) -
For your References section:
Engineering rules that minimize germline silencing of transgenes in simple extrachromosomal arrays in C. elegans. Aljohani MD, El Mouridi S, Priyadarshini M, Vargas-Velazquez AM, Frokjaer-Jensen C. Nat Commun. 2020 Dec 9;11(1):6300. doi: 10.1038/s41467-020-19898-0. 10.1038/s41467-020-19898-0 PubMed 33298957