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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15983 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFA6a-5FLAG-KanMX6
- Backbone size (bp) 4300
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- 5xFlag
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site See map (not destroyed)
- 3′ cloning site See map (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Template vector for a PCR based gene-tagging (5 x FLAG tag) method in S. pombe.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFA6a-5FLAG-KanMX6 was a gift from Eishi Noguchi (Addgene plasmid # 15983 ; http://n2t.net/addgene:15983 ; RRID:Addgene_15983) -
For your References section:
A vector system for genomic FLAG epitope-tagging in Schizosaccharomyces pombe. Noguchi C, Garabedian MV, Malik M, Noguchi E. Biotechnol J. 2008 Aug 26. ():. 10.1002/biot.200800140 PubMed 18729046
Map uploaded by the depositor.
