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Addgene

pAL-SK-swi3+
(Plasmid #15982)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15982 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAL-SK
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Swi3
  • Species
    S. pombe (fission yeast)
  • Insert Size (bp)
    1138
  • Entrez Gene
    swi3

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HincII/SmaI (destroyed during cloning)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAL-SK-swi3+ was a gift from Eishi Noguchi (Addgene plasmid # 15982 ; http://n2t.net/addgene:15982 ; RRID:Addgene_15982)

  • For your References section:

    Swi1 and Swi3 are components of a replication fork protection complex in fission yeast. Noguchi E, Noguchi C, McDonald WH, Yates JR, Russell P. Mol Cell Biol. 2004 Oct . 24(19):8342-55. 10.1128/MCB.24.19.8342-8355.2004 PubMed 15367656
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