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pMNK54 - piRNAi 21ur-1224 - him-5
(Plasmid #159818)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159818 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTwist+Amp+High+Copy
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    him-5 piRNAi 21ur-1224
  • Species
    C. elegans (nematode)
  • Mutation
    Not applicable

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See www.wormbuilder.org for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMNK54 - piRNAi 21ur-1224 - him-5 was a gift from Christian Froekjaer-Jensen (Addgene plasmid # 159818 ; http://n2t.net/addgene:159818 ; RRID:Addgene_159818)

  • For your References section:

    Reprogramming the piRNA pathway for multiplexed and transgenerational gene silencing in C. elegans. Priyadarshini M, Ni JZ, Vargas-Velazquez AM, Gu SG, Frokjaer-Jensen C. Nat Methods. 2022 Feb;19(2):187-194. doi: 10.1038/s41592-021-01369-z. Epub 2022 Feb 3. 10.1038/s41592-021-01369-z PubMed 35115715
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