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Addgene

pDblet-swi1+
(Plasmid #15981)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15981 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDblet
  • Backbone size w/o insert (bp) 6200
  • Vector type
    Yeast Expression
  • Selectable markers
    URA4 (S. pombe equivalent to URA3)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Swi1
  • Species
    S. pombe (fission yeast)
  • Insert Size (bp)
    3678
  • Entrez Gene
    swi1 (a.k.a. SPBC216.06c)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer M13R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDblet-swi1+ was a gift from Eishi Noguchi (Addgene plasmid # 15981 ; http://n2t.net/addgene:15981 ; RRID:Addgene_15981)

  • For your References section:

    Swi1 prevents replication fork collapse and controls checkpoint kinase Cds1. Noguchi E, Noguchi C, Du LL, Russell P. Mol Cell Biol. 2003 Nov . 23(21):7861-74. 10.1128/MCB.23.21.7861-7874.2003 PubMed 14560029
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