pAL-SK-swi1+
(Plasmid
#15980)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 15980 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAL-SK
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSwi1
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SpeciesS. pombe (fission yeast)
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Insert Size (bp)3678
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Entrez Geneswi1 (a.k.a. SPBC216.06c)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAL-SK-swi1+ was a gift from Eishi Noguchi (Addgene plasmid # 15980 ; http://n2t.net/addgene:15980 ; RRID:Addgene_15980) -
For your References section:
Swi1 prevents replication fork collapse and controls checkpoint kinase Cds1. Noguchi E, Noguchi C, Du LL, Russell P. Mol Cell Biol. 2003 Nov . 23(21):7861-74. 10.1128/MCB.23.21.7861-7874.2003 PubMed 14560029