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pSEM234 - pEXP[Pmlc-1 | mCherry(2xNLS) | cbr-tbb-2 UTR]
(Plasmid #159795)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159795 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST R4-R3
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pEXP[Pmlc-1 | mCherry(2xNLS) | cbr-tbb-2 UTR]
  • Species
    C. elegans (nematode)
  • Mutation
    Not applicable

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See www.wormbuilder.org for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSEM234 - pEXP[Pmlc-1 | mCherry(2xNLS) | cbr-tbb-2 UTR] was a gift from Christian Froekjaer-Jensen (Addgene plasmid # 159795 ; http://n2t.net/addgene:159795 ; RRID:Addgene_159795)

  • For your References section:

    Characterizing a strong pan-muscular promoter (Pmlc-1) as a fluorescent co-injection marker to select for single-copy insertions. El Mouridi S, Peng Y, Frokjaer-Jensen C. MicroPubl Biol. 2020 Sep 3;2020. doi: 10.17912/micropub.biology.000302. 10.17912/micropub.biology.000302 PubMed 32908967
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