pGL3-U6-pegRNA_KCNA1-EGFP
(Plasmid #159787)

• Purpose: S. pyogenes pegRNA for C to A mutation at the KCNA1 site of human cells using prime editing
• Depositing Lab: Xiaolong Wang
• Publication: Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1038/s41421-020-0165-z. eCollection 2020.

Backbone

• Vector backbone: pGL3-U6-sgRNA-EGFP
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: spacer of pegRNA targeting KCNA1 gene
• Species: H. sapiens (human)
• Entrez Gene: KCNA1 (a.k.a. AEMK, EA1, HBK1, HUK1, KV1.1, MBK1, MK1, RBK1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • PGL3-U6-pegRNA_KCNA1-EGFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Other insert name: RT&PBS of pegRNA targting KCNA1 gene