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pGL3-U6-pegRNA_SRD5A3-EGFP
(Plasmid #159781)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159781 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3-U6-sgRNA-EGFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    spacer of pegRNA targeting SRD5A3 gene
  • Species
    H. sapiens (human)
  • Entrez Gene
    SRD5A3 (a.k.a. CDG1P, CDG1Q, SRD5A2L, FLJ13352, SRD5A2L1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Other insert name: RT&PBS of pegRNA targting SRD5A3 gene

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-U6-pegRNA_SRD5A3-EGFP was a gift from Xiaolong Wang (Addgene plasmid # 159781 ; http://n2t.net/addgene:159781 ; RRID:Addgene_159781)

  • For your References section:

    Efficient generation of mouse models with the prime editing system. Liu Y, Li X, He S, Huang S, Li C, Chen Y, Liu Z, Huang X, Wang X. Cell Discov. 2020 Apr 28;6:27. doi: 10.1038/s41421-020-0165-z. eCollection 2020. 10.1038/s41421-020-0165-z PubMed 32351707
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