pAS404
(Plasmid #15978)

• Purpose: (Empty Backbone)
• Depositing Lab: Eishi Noguchi
• Publication: Nakashima et al Genetics. 1999 Jul . 152(3):853-67.

Backbone

• Vector backbone: pAS404
• Backbone size (bp): 5465
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: See map (not destroyed)
• 3′ cloning site: See map (not destroyed)
• 5′ sequencing primer: Gal4-Nterm

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

S. cerevisiae-E. coli shuttle vector. Cloning vector for two-hybrid system to express a GAL4 DBD-HA hybrid protein under the control of the ADH1 promoter. The 1.2-kb SacI/SalI fragment of pAS1 containing the ADH promoter, the GAL4 DNA-binding domain, and the HA epitope was inserted into the SacI/SalI sites of pRS404. Multi cloning sites from pBluescript II, f1(+) from pBluescript II.