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Addgene

pCVD293
(Plasmid #159765)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159765 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFUSE
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ACE2
  • Species
    H. sapiens (human)
  • Mutation
    K31F/H34I/E35Q
  • Entrez Gene
    ACE2 (a.k.a. ACEH)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2020.07.31.231746 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCVD293 was a gift from Jim Wells (Addgene plasmid # 159765 ; http://n2t.net/addgene:159765 ; RRID:Addgene_159765)

  • For your References section:

    Engineered ACE2 receptor traps potently neutralize SARS-CoV-2. Glasgow A, Glasgow J, Limonta D, Solomon P, Lui I, Zhang Y, Nix MA, Rettko NJ, Zha S, Yamin R, Kao K, Rosenberg OS, Ravetch JV, Wiita AP, Leung KK, Lim SA, Zhou XX, Hobman TC, Kortemme T, Wells JA. Proc Natl Acad Sci U S A. 2020 Nov 10;117(45):28046-28055. doi: 10.1073/pnas.2016093117. Epub 2020 Oct 22. 10.1073/pnas.2016093117 PubMed 33093202
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