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Addgene

pAM1479a3
(Plasmid #159725)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159725 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGBKCg
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mDHFR
  • Species
    M. musculus (mouse)
  • Mutation
    remove start and stop codon of Dhfr
  • Entrez Gene
    Dhfr (a.k.a. 8430436I03Rik)
  • Promoter ADH1
  • Tags / Fusion Proteins
    • MYITV-GGGGGG (N terminal on insert)
    • Gal4DBD (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Lab collection

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For 3' clonging site, a XhoI site that is present naturally in the sequence of a segment encoding the Gal4 DBD.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM1479a3 was a gift from Alexander Varshavsky (Addgene plasmid # 159725 ; http://n2t.net/addgene:159725 ; RRID:Addgene_159725)

  • For your References section:

    Gid10 as an alternative N-recognin of the Pro/N-degron pathway. Melnykov A, Chen SJ, Varshavsky A. Proc Natl Acad Sci U S A. 2019 Aug 6;116(32):15914-15923. doi: 10.1073/pnas.1908304116. Epub 2019 Jul 23. 10.1073/pnas.1908304116 PubMed 31337681
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