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Addgene

pCSJ182
(Plasmid #159723)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159723 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGADCg
  • Vector type
    Yeast Expression ; Yeast two-hybrid assay
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GID4
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1086
  • Mutation
    remove stop codon
  • GenBank ID
  • Entrez Gene
    VID24 (a.k.a. YBR105C, GID4)
  • Promoter ADH1
  • Tags / Fusion Proteins
    • 3xflag (C terminal on insert)
    • Gal4AD (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCSJ182 was a gift from Alexander Varshavsky (Addgene plasmid # 159723 ; http://n2t.net/addgene:159723 ; RRID:Addgene_159723)

  • For your References section:

    An N-end rule pathway that recognizes proline and destroys gluconeogenic enzymes. Chen SJ, Wu X, Wadas B, Oh JH, Varshavsky A. Science. 2017 Jan 27;355(6323). pii: 355/6323/eaal3655. doi: 10.1126/science.aal3655. 10.1126/science.aal3655 PubMed 28126757
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