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Addgene

pCSJ392
(Plasmid #159722)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159722 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGADCg
  • Vector type
    Yeast Expression ; Yeast two-hybrid assay
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GID10
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    remove stop codon
  • Entrez Gene
    GID10 (a.k.a. YGR066C)
  • Promoter ADH1
  • Tags / Fusion Proteins
    • 3xflag (C terminal on insert)
    • Gal4AD (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCSJ392 was a gift from Alexander Varshavsky (Addgene plasmid # 159722 ; http://n2t.net/addgene:159722 ; RRID:Addgene_159722)

  • For your References section:

    Gid10 as an alternative N-recognin of the Pro/N-degron pathway. Melnykov A, Chen SJ, Varshavsky A. Proc Natl Acad Sci U S A. 2019 Aug 6;116(32):15914-15923. doi: 10.1073/pnas.1908304116. Epub 2019 Jul 23. 10.1073/pnas.1908304116 PubMed 31337681
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