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pOmnibac_ZZ_linker_K490_GFP_SNAP
(Plasmid #159721)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159721 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOmnibac
  • Total vector size (bp) 7806
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    KIF5B (only amino acids 1-490), K490
  • Alt name
    Kinesin-1 heavy chain
  • Alt name
    UniProtKB - P33176 (KINH_HUMAN), P33176-1
  • Species
    H. sapiens (human)
  • Mutation
    Only amino acids (1-490)
  • GenBank ID
    3799
  • Entrez Gene
    KIF5B (a.k.a. HEL-S-61, KINH, KNS, KNS1, UKHC)
  • Tags / Fusion Proteins
    • ZZ affinity tag (N terminal on insert)
    • GFP and SNAP tags (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOmnibac_ZZ_linker_K490_GFP_SNAP was a gift from Ahmet Yildiz (Addgene plasmid # 159721 ; http://n2t.net/addgene:159721 ; RRID:Addgene_159721)

  • For your References section:

    Structural and functional insight into regulation of kinesin-1 by microtubule-associated protein MAP7. Ferro LS, Fang Q, Eshun-Wilson L, Fernandes J, Jack A, Farrell DP, Golcuk M, Huijben T, Costa K, Gur M, DiMaio F, Nogales E, Yildiz A. Science. 2022 Jan 21;375(6578):326-331. doi: 10.1126/science.abf6154. Epub 2022 Jan 20. 10.1126/science.abf6154 PubMed 35050657
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