pOmnibac_ZZ_linker_ybbR_Tau MTBD
(Plasmid #159719)

• Purpose: Expresses microtubule-binding domain of tau (amino acids 242-367) in Sf9 cells with a ZZ affinity tag and a ybbR tag
• Depositing Lab: Ahmet Yildiz
• Publication: Ferro et al Science. 2022 Jan 21;375(6578):326-331. doi: 10.1126/science.abf6154. Epub 2022 Jan 20.

Backbone

• Vector backbone: pOmnibac
• Total vector size (bp): 5478
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Tau microtubule-binding domain only (amino acids 242-367)
• Alt name: Tau, MAPT, Isoform Tau-F (identifier: P10636-8)
• Alt name: Microtubule-associated protein tau
• Alt name: UniProtKB - P10636 (TAU_HUMAN)
• Species: H. sapiens (human)
• Mutation: Only amino acids 242-367
• Entrez Gene: MAPT (a.k.a. DDPAC, FTD1, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40)
• Tag / Fusion Protein:
  • ZZ affinity tag, ybbR tag

Resource Information

• Supplemental Documents:
  • N-ybbR MTBD only Tau pOmniBac.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry