pG20_Myc_Blp
(Plasmid
#159716)
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Purpose(Empty Backbone) pGREEN-based cloning construct for expression in planta
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 159716 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGREEN
- Backbone size (bp) 5077
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Vector typePlant Expression
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Selectable markersBasta
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Tag
/ Fusion Protein
- Myc (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- Myc (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Empty pGREEN expression vector for cloning; HSP terminator
Addgene QC identified an Insertion element (IS4) present in the backbone that does not appear to have a functional impact in its current location. Requesting scientists should confirm the expected function of plasmid and location of IS4 element.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pG20_Myc_Blp was a gift from Hans-Henning Kunz (Addgene plasmid # 159716 ; http://n2t.net/addgene:159716 ; RRID:Addgene_159716) -
For your References section:
An updated pGREEN-based vector suite for cost-effective cloning in plant molecular biology. Pratt AI, Knoblauch J, Kunz HH. MicroPubl Biol. 2020 Oct 7;2020. doi: 10.17912/micropub.biology.000317. 10.17912/micropub.biology.000317 PubMed 33274321