pG20_GUS_Blp
(Plasmid
#159706)
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Purpose(Empty Backbone) pGREEN-based cloning construct for expression in planta
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 159706 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGREEN
- Backbone size (bp) 6856
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Vector typePlant Expression
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Selectable markersBasta
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Tag
/ Fusion Protein
- GUS (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- GUS (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Empty pGREEN expression vector for cloning; HSP terminator. Addgene QC identified an IS4 element in the plasmid backbone that the depositor does not find to be of functional concern but care should be taken to ensure the IS4 element has not transposed elsewhere.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pG20_GUS_Blp was a gift from Hans-Henning Kunz (Addgene plasmid # 159706 ; http://n2t.net/addgene:159706 ; RRID:Addgene_159706) -
For your References section:
An updated pGREEN-based vector suite for cost-effective cloning in plant molecular biology. Pratt AI, Knoblauch J, Kunz HH. MicroPubl Biol. 2020 Oct 7;2020. doi: 10.17912/micropub.biology.000317. 10.17912/micropub.biology.000317 PubMed 33274321
