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R726-M01-892: His6-Halotag-tev-Hs.RAF1(52-131)
(Plasmid #159696)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159696 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDest-892
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    His6-Halotag-tev-Hs.RAF1(52-131)
  • Alt name
    RAF1 RBD
  • Species
    H. sapiens (human)
  • Entrez Gene
    RAF1 (a.k.a. CMD1NN, CRAF, NS5, Raf-1, c-Raf)
  • Tag / Fusion Protein
    • His6-Halotag-tev (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    R726-M01-892: His6-Halotag-tev-Hs.RAF1(52-131) was a gift from Dominic Esposito (Addgene plasmid # 159696 ; http://n2t.net/addgene:159696 ; RRID:Addgene_159696)

  • For your References section:

    Quantitative biophysical analysis defines key components modulating recruitment of the GTPase KRAS to the plasma membrane. Lakshman B, Messing S, Schmid EM, Clogston JD, Gillette WK, Esposito D, Kessing B, Fletcher DA, Nissley DV, McCormick F, Stephen AG, Jean-Francois FL. J Biol Chem. 2019 Feb 8;294(6):2193-2207. doi: 10.1074/jbc.RA118.005669. Epub 2018 Dec 17. 10.1074/jbc.RA118.005669 PubMed 30559287
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