Skip to main content
Addgene

R989-X79-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) M188L
(Plasmid #159692)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159692 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pDest-636
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    His6-MBP-tev-G-Hs.KRAS4b(1-188) M188L
  • Species
    H. sapiens (human)
  • Mutation
    M188L
  • Entrez Gene
    KRAS (a.k.a. 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2)
  • Tag / Fusion Protein
    • His6-MBP-tev (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    R989-X79-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) M188L was a gift from Dominic Esposito (Addgene plasmid # 159692 ; http://n2t.net/addgene:159692 ; RRID:Addgene_159692)

  • For your References section:

    Production of authentic geranylgeranylated KRAS4b using an engineered baculovirus system. Procter L, Grose C, Esposito D. Protein Expr Purif. 2018 Nov;151:99-105. doi: 10.1016/j.pep.2018.06.012. Epub 2018 Jun 22. 10.1016/j.pep.2018.06.012 PubMed 29936133
Related items:
Commonly requested with: