R979-X57-008: Hs.KRAS4b(1-169)
(Plasmid
#159690)
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PurposeBaculovirus protein expression of KRAS4b (1-169)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 159690 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDest-8
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHs.KRAS4b(1-169)
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SpeciesH. sapiens (human)
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Entrez GeneKRAS (a.k.a. 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
R979-X57-008: Hs.KRAS4b(1-169) was a gift from Dominic Esposito (Addgene plasmid # 159690 ; http://n2t.net/addgene:159690 ; RRID:Addgene_159690) -
For your References section:
Structures of N-terminally processed KRAS provide insight into the role of N-acetylation. Dharmaiah S, Tran TH, Messing S, Agamasu C, Gillette WK, Yan W, Waybright T, Alexander P, Esposito D, Nissley DV, McCormick F, Stephen AG, Simanshu DK. Sci Rep. 2019 Jul 19;9(1):10512. doi: 10.1038/s41598-019-46846-w. 10.1038/s41598-019-46846-w PubMed 31324887