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R702-X31-566: His6-MBP-tev-G-Hs.PDE6D(2-150)
(Plasmid #159689)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159689 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDest-566
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    His6-MBP-tev-G-Hs.PDE6D(2-150)
  • Alt name
    PDE-delta
  • Species
    H. sapiens (human)
  • Entrez Gene
    PDE6D (a.k.a. JBTS22, PDED)
  • Tag / Fusion Protein
    • His6-MBP-tev (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    R702-X31-566: His6-MBP-tev-G-Hs.PDE6D(2-150) was a gift from Dominic Esposito (Addgene plasmid # 159689 ; http://n2t.net/addgene:159689 ; RRID:Addgene_159689)

  • For your References section:

    Structural basis of recognition of farnesylated and methylated KRAS4b by PDEdelta. Dharmaiah S, Bindu L, Tran TH, Gillette WK, Frank PH, Ghirlando R, Nissley DV, Esposito D, McCormick F, Stephen AG, Simanshu DK. Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):E6766-E6775. doi: 10.1073/pnas.1615316113. Epub 2016 Oct 17. 10.1073/pnas.1615316113 PubMed 27791178
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