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Addgene

R713-M91-303: CMV51p> Halotag-Hs.NRAS
(Plasmid #159682)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159682 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDest-303
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Halotag-Hs.NRAS
  • Species
    H. sapiens (human)
  • Entrez Gene
    NRAS (a.k.a. ALPS4, CMNS, KRAS, N-ras, NCMS, NRAS1, NS6)
  • Tag / Fusion Protein
    • Halotag (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    R713-M91-303: CMV51p> Halotag-Hs.NRAS was a gift from Dominic Esposito (Addgene plasmid # 159682 ; http://n2t.net/addgene:159682 ; RRID:Addgene_159682)

  • For your References section:

    Membrane interactions of the globular domain and the hypervariable region of KRAS4b define its unique diffusion behavior. Goswami D, Chen, Yang Y, Gudla PR, Columbus J, Worthy K, Rigby M, Wheeler M, Mukhopadhyay S, Powell K, Burgan W, Wall V, Esposito D, Simanshu DK, Lightstone FC, Nissley DV, McCormick F, Turbyville T. Elife. 2020 Jan 20;9. pii: 47654. doi: 10.7554/eLife.47654. 10.7554/eLife.47654 PubMed 31958057
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