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Addgene

p405TEF1
(Plasmid #15968)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15968 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p405GALS
  • Backbone manufacturer
    N. Buchler
  • Backbone size w/o insert (bp) 5074
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TEF1 promoter + polylinker + CYC1 terminator
  • Alt name
    Translational elongation factor EF-1 alpha
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1060
  • Mutation
    TEF1 promoter is between SacI and XbaI restriction sites.
  • Entrez Gene
    TEF1 (a.k.a. YPR080W)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site NgoMIV (not destroyed)
  • 5′ sequencing primer M13 Reverse
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Insert was cut from p404TEF1, which was obtained from Ben Timney.
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p405TEF1 was a gift from Nicolas Buchler & Fred Cross (Addgene plasmid # 15968 ; http://n2t.net/addgene:15968 ; RRID:Addgene_15968)
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