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874V=βTub-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40
(Plasmid #159672)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159672 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBac
  • Backbone size w/o insert (bp) 8000
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    βTub-NLS-hSpCas9-T2A-GFP
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    6200
  • GenBank ID
    betaTub85D (CG9359) promoter
  • Tag / Fusion Protein
    • T2A-GFP (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    874V=βTub-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40 was a gift from Omar Akbari (Addgene plasmid # 159672 ; http://n2t.net/addgene:159672 ; RRID:Addgene_159672)

  • For your References section:

    A confinable home and rescue gene drive for population modification. Kandul NP, Liu J, Bennett JB, Marshall JM, Akbari OS. Elife. 2021 Mar 5;10. pii: 65939. doi: 10.7554/eLife.65939. 10.7554/eLife.65939 PubMed 33666174
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