Sticky-349-3X
(Plasmid
#159643)
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Purpose(Empty Backbone) Non-coding DNA sequence for dinucleosome preparation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 159643 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
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Backbone manufacturerGenscript
- Backbone size (bp) 2710
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Modifications to backbonecloned into the HindIII and BamHI sites of the pUC19 vector
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Vector typeBacterial vector for DNA production
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13 fwd
- 3′ sequencing primer M13 rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please cite the following article in any publications where this construct is utilized:
Adhireksan Z, Sharma D, Lee PL, Davey CA
Near-atomic resolution structures of interdigitated nucleosome fibres.
Nat Commun. 2020 Sep 21;11(1):4747. doi: 10.1038/s41467-020-18533-2.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Sticky-349-3X was a gift from Curt Davey (Addgene plasmid # 159643 ; http://n2t.net/addgene:159643 ; RRID:Addgene_159643) -
For your References section:
Near-atomic resolution structures of interdigitated nucleosome fibres. Adhireksan Z, Sharma D, Lee PL, Davey CA. Nat Commun. 2020 Sep 21;11(1):4747. doi: 10.1038/s41467-020-18533-2. 10.1038/s41467-020-18533-2 PubMed 32958761