Integrin-αv Ectodomain M400C
(Plasmid
#159642)
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PurposeContains integrin alpha v residues 1–960 with the M400C mutation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 159642 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1 hygro
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 8300
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameIntegrin alpha v ectodomain
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Alt nameav ectodomain
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Insert Size (bp)2800
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MutationM400C
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7 forward
- 3′ sequencing primer T7 reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Integrin-αv Ectodomain M400C was a gift from Timothy Springer (Addgene plasmid # 159642 ; http://n2t.net/addgene:159642 ; RRID:Addgene_159642) -
For your References section:
Atypical interactions of integrin alphaVbeta8 with pro-TGF-beta1. Wang J, Dong X, Zhao B, Li J, Lu C, Springer TA. Proc Natl Acad Sci U S A. 2017 May 23;114(21):E4168-E4174. doi: 10.1073/pnas.1705129114. Epub 2017 May 8. 10.1073/pnas.1705129114 PubMed 28484027