p405GALL
(Plasmid
#15964)
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Depositing Labs
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15964 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS405
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Backbone manufacturerSikorski & Hieter, Genetics 122:19-27 (1989)
- Backbone size w/o insert (bp) 3814
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Vector typeBacterial Expression, Yeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep405GALL
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Alt nametruncated GAL1 (long) promoter
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Alt nameGalactokinase
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)2353
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MutationGALL promoter is between SacI and XbaI restriction sites.
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Entrez GeneGAL1 (a.k.a. YBR020W)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglI (not destroyed)
- 3′ cloning site BglI (not destroyed)
- 5′ sequencing primer M13 Reverse (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byInsert was cut from Mumberg plasmid p415GALL, which was obtained from ATCC.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Mumberg D, Muller R, Funk M. Nucleic Acids Res. 25:5767-8 (1994).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p405GALL was a gift from Nicolas Buchler & Fred Cross (Addgene plasmid # 15964 ; http://n2t.net/addgene:15964 ; RRID:Addgene_15964)
