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Addgene

Integrin-β6 Headpiece I270C
(Plasmid #159638)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159638 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ET10
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 6800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Integrin beta 6 headpiece I270C
  • Alt name
    b6 headpiece I270C
  • Insert Size (bp)
    1300
  • Mutation
    I270C
  • Tag / Fusion Protein
    • 6x His (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer AGTGGGGTGTTGGGG
  • 3′ sequencing primer GGCTACGGCCTCCAAACCCTCTTCCAA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Integrin-β6 Headpiece I270C was a gift from Timothy Springer (Addgene plasmid # 159638 ; http://n2t.net/addgene:159638 ; RRID:Addgene_159638)

  • For your References section:

    Structural determinants of integrin beta-subunit specificity for latent TGF-beta. Dong X, Hudson NE, Lu C, Springer TA. Nat Struct Mol Biol. 2014 Dec;21(12):1091-6. doi: 10.1038/nsmb.2905. Epub 2014 Nov 10. 10.1038/nsmb.2905 PubMed 25383667
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