Integrin-αv Headpiece M400GC
(Plasmid #159637)

• Purpose: Contains integrin alpha v residues 1–594 with the M400C mutation and Gly inserted prior to residue 400
• Depositing Lab: Timothy Springer
• Publication: Dong et al Nat Struct Mol Biol. 2014 Dec;21(12):1091-6. doi: 10.1038/nsmb.2905. Epub 2014 Nov 10.

Backbone

• Vector backbone: pcDNA3.1 hygro
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 7300
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: alpha v headpiece M400GC
• Alt name: av-hp (M400C)
• Insert Size (bp): 1800
• Mutation: M400C
• Tags / Fusion Proteins:
  • Strep II (C terminal on insert)
  • 6x His (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7 forward
• 3′ sequencing primer: T7 reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry