Syt1LeftArm-3XGS-GCaMP6s-SV40-3XP3-dsRed-SV40-Syt1RightArm
(Plasmid
#159636)
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PurposeDonor plasmid for generating Syt1:GCaMP6s strain in Aedes aegypti with CRISPR/Cas9
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 159636 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepsL1180
- Backbone size w/o insert (bp) 3422
- Total vector size (bp) 9570
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGCaMP6s
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SpeciesAedes aegypti
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Insert Size (bp)1353
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Syt1LeftArm-3XGS-GCaMP6s-SV40-3XP3-dsRed-SV40-Syt1RightArm was a gift from Carolyn McBride (Addgene plasmid # 159636 ; http://n2t.net/addgene:159636 ; RRID:Addgene_159636) -
For your References section:
Development of a pan-neuronal genetic driver in Aedes aegypti mosquitoes. Zhao Z, Tian D, McBride CS. Cell Rep Methods. 2021 Jul 26;1(3):100042. doi: 10.1016/j.crmeth.2021.100042. Epub 2021 Jun 30. 10.1016/j.crmeth.2021.100042 PubMed 34590074