pJo55
(Plasmid
#159623)
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Purpose(Empty Backbone) An empty HIS3 plasmid for translation factor eIF4E complementation test in yeast, strain Jo55. Golden Gate cloning using BsaI. Selection with RFP after cloning.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 159623 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAV123
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Backbone manufacturerhttps://www.addgene.org/63189/
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Vector typeBacterial Expression, Yeast Expression
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJo55 was a gift from Vasiliy Taranov (Addgene plasmid # 159623 ; http://n2t.net/addgene:159623 ; RRID:Addgene_159623) -
For your References section:
VPg of Potato Virus Y and Potato Cap-Binding eIF4E Factors: Selective Interaction and Its Supposed Mechanism. Lebedeva MV, Nikonova EY, Terentiev AA, Taranov VV, Babakov AV, Nikonov OS. Biochemistry (Mosc). 2021 Sep;86(9):1128-1138. doi: 10.1134/S000629792109008X. 10.1134/S000629792109008X PubMed 34565316
