p405MET3
(Plasmid #15962)

• Depositing Labs: Nicolas Buchler, Fred Cross
• Publication: Buchler lab plasmids (unpublished)

Backbone

• Vector backbone: p405MET3b
• Backbone manufacturer: A. Geoghegan (Rockefeller University)
• Backbone size w/o insert (bp): 5578
• Vector type: Bacterial Expression, Yeast Expression
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MET3 promoter + polylinker + CYC1 terminator
• Alt name: ATP sulfurylase
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 649
• Mutation: MET3 promoter is between SacI and XbaI restriction sites.
• Entrez Gene: MET3

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: NgoMIV (not destroyed)
• 5′ sequencing primer: M13 Reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Original vector p405MET3b was created by subcloning PCR fragment of genomic MET3 promoter (with SacI and XbaI restriction sites introduced) into SacI-XbaI cut vector pRS405. This plasmid does not contains CYC1 terminator. Plasmid p405MET3 was created by subcloning XbaI-NgoMIV insert from p405GALS (insert = polylinker + CYC1 terminator) into XbaI-NgoMIV cut p405MET3b vector.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry