COVID-SARS2 NSP10
(Plasmid
#159616)
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PurposeBacterial expression plasmid for COVID-SARS2 NSP10 fused to GST
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 159616 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepNIC-CTH0
- Backbone size w/o insert (bp) 5340
- Total vector size (bp) 6455
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCovid-SARS2 Nsp10
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SpeciesSynthetic; COVID-SARS2
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Insert Size (bp)1113
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GenBank IDNC_045512.2
- Promoter T7 - LacO
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Tag
/ Fusion Protein
- GST-3C protease site (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7F
- 3′ sequencing primer T7R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
COVID-SARS2 NSP10 was a gift from Opher Gileadi (Addgene plasmid # 159616 ; http://n2t.net/addgene:159616 ; RRID:Addgene_159616)
