COVID-SARS2 NSP16/10
(Plasmid #159615)

• Purpose: Bacterial co-expression vector fo Covid-SARS2 NSP16 and NSP10
• Depositing Lab: Opher Gileadi
• Publication: Bacterial co-expression vector for Covid-SARS2 NSP16 and NSP10 (unpublished)

Backbone

• Vector backbone: pNIC-ZB
• Backbone manufacturer: Addgene Plasmid #26107
• Backbone size w/o insert (bp): 5515
• Total vector size (bp): 6862
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: Covid-SARS2 Nsp16
• Species: Synthetic; COVID-SARS2
• Insert Size (bp): 900
• Mutation: Codon-optimized for E. coli expression
• GenBank ID: NC_045512.2
• Promoter: T7 - LacO
• Tag / Fusion Protein:
  • His6-Zb-TEV (N terminal on backbone)

Cloning Information for Gene/Insert 1

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7F
• 3′ sequencing primer: custom

Gene/Insert 2

• Gene/Insert name: Covid-SARS2 Nsp10
• Species: Synthetic; COVID-SARS2
• Mutation: Codon-optimized for E. coli expression
• GenBank ID: NC_045512.2
• Promoter: (Bicistronic)
• Tag / Fusion Protein:
  • none

Cloning Information for Gene/Insert 2

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: custom
• 3′ sequencing primer: T7R

Resource Information

• Supplemental Documents:
  • COVIDnsp16-10.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry