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Addgene

COVID-SARS2 NSP13
(Plasmid #159614)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159614 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNIC-ZB
  • Backbone manufacturer
    Addgene Plasmid #26107
  • Backbone size w/o insert (bp) 5515
  • Total vector size (bp) 7327
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Covid-SARS2 Nsp13
  • Species
    Synthetic; COVID-SARS2
  • Insert Size (bp)
    1809
  • Mutation
    Codon-optimized for E. coli expression
  • GenBank ID
    NC_045512.2
  • Promoter T7 - LacO
  • Tag / Fusion Protein
    • His6-Zb-TEV (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    COVID-SARS2 NSP13 was a gift from Opher Gileadi (Addgene plasmid # 159614 ; http://n2t.net/addgene:159614 ; RRID:Addgene_159614)

  • For your References section:

    Structure, mechanism and crystallographic fragment screening of the SARS-CoV-2 NSP13 helicase. Newman JA, Douangamath A, Yadzani S, Yosaatmadja Y, Aimon A, Brandao-Neto J, Dunnett L, Gorrie-Stone T, Skyner R, Fearon D, Schapira M, von Delft F, Gileadi O. Nat Commun. 2021 Aug 11;12(1):4848. doi: 10.1038/s41467-021-25166-6. 10.1038/s41467-021-25166-6 PubMed 34381037
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