COVID-SARS2 NSP14/10
(Plasmid #159613)

• Purpose: Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10
• Depositing Lab: Opher Gileadi
• Publication: Baddock et al Nucleic Acids Res. 2022 Jan 17. pii: 6509090. doi: 10.1093/nar/gkab1303.

Backbone

• Vector backbone: pNIC28-Bsa4
• Backbone manufacturer: Addgene Plasmid #26103
• Backbone size w/o insert (bp): 5356
• Total vector size (bp): 7387
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: Covid-SARS2 Nsp14
• Species: Synthetic; COVID-SARS2
• Insert Size (bp): 1653
• Mutation: Codon-optimized for E. coli expression
• GenBank ID: NC_045512.2
• Entrez Gene: ORF1ab (a.k.a. GU280_gp01)
• Promoter: T7 - LacO
• Tag / Fusion Protein:
  • His6, TEV cleavage site (N terminal on backbone)

Cloning Information for Gene/Insert 1

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7F
• 3′ sequencing primer: custom

Gene/Insert 2

• Gene/Insert name: Covid-SARS2 Nsp10
• Species: Synthetic; COVID-SARS2
• Insert Size (bp): 423
• Mutation: Codon-optimized for E. coli expression
• GenBank ID: NC_045512.2
• Promoter: (Bicistronic)
• Tag / Fusion Protein:
  • none

Cloning Information for Gene/Insert 2

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: custom
• 3′ sequencing primer: T7R

Resource Information

• Supplemental Documents:
  • COVIDnsp14-10.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry