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Addgene

EC71022
(Plasmid #159608)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159608 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    EC10161
  • Backbone size w/o insert (bp) 2426
  • Total vector size (bp) 3437
  • Vector type
    Cre/Lox, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    loxP-mCherry-HDEL-t35S-loxP
  • Alt name
    L0.5_U-LoxP-mCherry-HDEL-t35S-LoxP
  • Species
    Synthetic
  • Insert Size (bp)
    1003
  • Mutation
    All BsaI, Esp3I and BPiI restriction sites were removed using synonymous changes

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer GTAAAACGACGGCCAG
  • 3′ sequencing primer AAGCGGGCAGTGAAAGGAAGGC
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EC71022 was a gift from Cristobal Uauy (Addgene plasmid # 159608 ; http://n2t.net/addgene:159608 ; RRID:Addgene_159608)

  • For your References section:

    A heat-shock inducible system for flexible gene expression in cereals. Harrington SA, Backhaus AE, Fox S, Rogers C, Borrill P, Uauy C, Richardson A. Plant Methods. 2020 Oct 14;16:137. doi: 10.1186/s13007-020-00677-3. eCollection 2020. 10.1186/s13007-020-00677-3 PubMed 33072173
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