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Addgene

pSF11-wNIR-GECO2-T2A-HO1
(Plasmid #159606)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159606 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSF11
  • Backbone size w/o insert (bp) 6090
  • Total vector size (bp) 8508
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    wNIR-GECO2-T2A-HO1
  • Alt name
    Codon optimized NIR-GECO2 and HO1 for worm expression
  • Species
    Synthetic
  • Insert Size (bp)
    2418
  • Promoter tag-168

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ctagaggatccccgggattgg
  • 3′ sequencing primer tgttgaagagtaattggacttagaagt
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSF11-wNIR-GECO2-T2A-HO1 was a gift from Robert Campbell (Addgene plasmid # 159606 ; http://n2t.net/addgene:159606 ; RRID:Addgene_159606)

  • For your References section:

    Improved genetically encoded near-infrared fluorescent calcium ion indicators for in vivo imaging. Qian Y, Cosio DMO, Piatkevich KD, Aufmkolk S, Su WC, Celiker OT, Schohl A, Murdock MH, Aggarwal A, Chang YF, Wiseman PW, Ruthazer ES, Boyden ES, Campbell RE. PLoS Biol. 2020 Nov 24;18(11):e3000965. doi: 10.1371/journal.pbio.3000965. eCollection 2020 Nov. 10.1371/journal.pbio.3000965 PubMed 33232322
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