pWC2
(Plasmid #15960)

• Depositing Lab: Jeff Gelles
• Publication: Subramanian et al J Gen Physiol. 2007 Nov . 130(5):445-55.

Backbone

• Vector backbone: pT7-7
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Kinesin 1-401 BCCP his tag
• Alt name: K401-BIO-H6
• Alt name: kinesin heavy chain
• Species: D. melanogaster (fly); E. coli
• Mutation: amino acids 1-401
• GenBank ID: M24441
• Entrez Gene: Khc (a.k.a. Dmel_CG7765, 2R6, CG7765, DK, DKH, Dm KHC, DmK, DmKHC, Dmel\CG7765, Dmkin, KHC, KIF 5A, KIF5, KIF5B, KIN, Kif5, Kin, Kin-1, Kinesin, Kinesin-1, khc, kin, kinesin, kinesin-1, l(2)W12, l(2)k13219, l(2)k13314, l(2R)W12, pgs)
• Tags / Fusion Proteins:
  • BCCP (C terminal on insert)
  • His6 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: N/A

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: L. Goldstein, J. Cronan
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry