Addgene: pKP515 Skip to main content
Addgene

pKP515
(Plasmid #159597)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159597 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD24
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    KP1394
  • Growth instructions
    KP1394 is W3110 fepA::kan, recA::cat. Plasmids can also be maintained in DH5alpha.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    fepA
  • Species
    Escherichia coli
  • Mutation
    none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKP515 was a gift from Kathleen Postle (Addgene plasmid # 159597 ; http://n2t.net/addgene:159597 ; RRID:Addgene_159597)

  • For your References section:

    FepA with globular domain deletions lacks activity. Vakharia HL, Postle K. J Bacteriol. 2002 Oct;184(19):5508-12. doi: 10.1128/jb.184.19.5508-5512.2002. 10.1128/jb.184.19.5508-5512.2002 PubMed 12218040
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