Skip to main content
Addgene

pKP325
(Plasmid #159596)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159596 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pACYC184
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    KP1317
  • Growth instructions
    KP1317 is W3110 ∆(ana-trp), pcnB1, zad-981::mini-kan. Plasmids can also be maintained in DH5alpha.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tonB
  • Species
    Escherichia coli
  • Mutation
    none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKP325 was a gift from Kathleen Postle (Addgene plasmid # 159596 ; http://n2t.net/addgene:159596 ; RRID:Addgene_159596)

  • For your References section:

    Protonmotive force, ExbB and ligand-bound FepA drive conformational changes in TonB. Larsen RA, Thomas MG, Postle K. Mol Microbiol. 1999 Mar;31(6):1809-24. doi: 10.1046/j.1365-2958.1999.01317.x. 10.1046/j.1365-2958.1999.01317.x PubMed 10209752
Related items:
Commonly requested with: