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Addgene

Actin-OptoNB
(Plasmid #159595)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159595 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHR
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ActinNB with AsLOV2 at loop 5 with 62-666

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Actin-OptoNB was a gift from Jose Avalos & Jared Toettcher (Addgene plasmid # 159595 ; http://n2t.net/addgene:159595 ; RRID:Addgene_159595)

  • For your References section:

    Optogenetic control of protein binding using light-switchable nanobodies. Gil AA, Carrasco-Lopez C, Zhu L, Zhao EM, Ravindran PT, Wilson MZ, Goglia AG, Avalos JL, Toettcher JE. Nat Commun. 2020 Aug 13;11(1):4044. doi: 10.1038/s41467-020-17836-8. 10.1038/s41467-020-17836-8 PubMed 32792536
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