LaM8-GG15
(Plasmid
#159593)
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PurposeOpto-Nanobody
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Depositing Labs
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Sequence Information
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Sequences (2) — Accept Affinity Reagent Sequence Policy
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Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 159593 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBAD
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLaM8 with AsLOV2 at GG15
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LaM8-GG15 was a gift from Jose Avalos & Jared Toettcher (Addgene plasmid # 159593 ; http://n2t.net/addgene:159593 ; RRID:Addgene_159593) -
For your References section:
Optogenetic control of protein binding using light-switchable nanobodies. Gil AA, Carrasco-Lopez C, Zhu L, Zhao EM, Ravindran PT, Wilson MZ, Goglia AG, Avalos JL, Toettcher JE. Nat Commun. 2020 Aug 13;11(1):4044. doi: 10.1038/s41467-020-17836-8. 10.1038/s41467-020-17836-8 PubMed 32792536
