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pFRT-DHX36iso1E335A
(Plasmid #159587)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159587 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFRT
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DHX36
  • Alt name
    RHAU
  • Alt name
    DEAH-Box Helicase 36
  • Alt name
    Isoform 1
  • Species
    H. sapiens (human)
  • Mutation
    E335A
  • GenBank ID
    NM_020865.3
  • Entrez Gene
    DHX36 (a.k.a. DDX36, G4R1, MLEL1, RHAU)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG HA (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFRT-DHX36iso1E335A was a gift from Markus Hafner (Addgene plasmid # 159587 ; http://n2t.net/addgene:159587 ; RRID:Addgene_159587)

  • For your References section:

    DHX36 prevents the accumulation of translationally inactive mRNAs with G4-structures in untranslated regions. Sauer M, Juranek SA, Marks J, De Magis A, Kazemier HG, Hilbig D, Benhalevy D, Wang X, Hafner M, Paeschke K. Nat Commun. 2019 Jun 3;10(1):2421. doi: 10.1038/s41467-019-10432-5. 10.1038/s41467-019-10432-5 PubMed 31160600
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