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psiCHECK2-2A-3xFLAG-SBP-2A
(Plasmid #159584)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159584 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    psiCHECK2
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 6279
  • Total vector size (bp) 5698
  • Modifications to backbone
    Depletion of synthetic poly(A) signal and HSV-TK promoter
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    2A-3xFLAG-SBP-2A
  • Species
    Synthetic; Porcine teschovirus-1

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGCGTGCTGAAGAACGAGCAGGGAAGCGGAGCTACTAACT
  • 3′ sequencing primer CTTCTTAATGTTCTTAGCATCGGCAGGTCCAGGGTTCTCCT
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    EIF2S3 5UTR
  • Species
    H. sapiens (human)

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ATACGACTCACTATAGGTTTCCTTCCTCTTTTGGCAACATGGCTTCCAAGGTGTA
  • 3′ sequencing primer TACACCTTGGAAGCCATGTTGCCAAAAGAGGAAGGAAACCTATAGTGAGTCGTAT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    psiCHECK2-2A-3xFLAG-SBP-2A was a gift from Shintaro Iwasaki (Addgene plasmid # 159584 ; http://n2t.net/addgene:159584 ; RRID:Addgene_159584)

  • For your References section:

    Genome-wide Survey of Ribosome Collision. Han P, Shichino Y, Schneider-Poetsch T, Mito M, Hashimoto S, Udagawa T, Kohno K, Yoshida M, Mishima Y, Inada T, Iwasaki S. Cell Rep. 2020 May 5;31(5):107610. doi: 10.1016/j.celrep.2020.107610. 10.1016/j.celrep.2020.107610 PubMed 32375038
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