pDest-635
(Plasmid
#159582)
-
Purpose(Empty Backbone) Gateway Destination vector for protein expression in baculovirus
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 159582 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backboneN/A
-
Vector typeInsect Expression
-
Tag
/ Fusion Protein
- His6 (N terminal on insert)
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol and Gentamicin, 25 & 10 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)ccdB Survival
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNone
-
Tag
/ Fusion Protein
- His6 (N terminal on insert)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pDest-635 was a gift from Dominic Esposito (Addgene plasmid # 159582 ; http://n2t.net/addgene:159582 ; RRID:Addgene_159582) -
For your References section:
Structural Insights into the SPRED1-Neurofibromin-KRAS Complex and Disruption of SPRED1-Neurofibromin Interaction by Oncogenic EGFR. Yan W, Markegard E, Dharmaiah S, Urisman A, Drew M, Esposito D, Scheffzek K, Nissley DV, McCormick F, Simanshu DK. Cell Rep. 2020 Jul 21;32(3):107909. doi: 10.1016/j.celrep.2020.107909. 10.1016/j.celrep.2020.107909 PubMed 32697994
