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Addgene

R725-X09-635: His6-tev-Hs.SPRED1(13-125)
(Plasmid #159581)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159581 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDest-635
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    His6-tev-Hs.SPRED1(13-125)
  • Species
    H. sapiens (human)
  • Entrez Gene
    SPRED1 (a.k.a. LGSS, NFLS, PPP1R147, hSpred1, spred-1)
  • Tag / Fusion Protein
    • His6-tev (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

insert contains optimized DNA sequence

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    R725-X09-635: His6-tev-Hs.SPRED1(13-125) was a gift from Dominic Esposito (Addgene plasmid # 159581 ; http://n2t.net/addgene:159581 ; RRID:Addgene_159581)

  • For your References section:

    Structural Insights into the SPRED1-Neurofibromin-KRAS Complex and Disruption of SPRED1-Neurofibromin Interaction by Oncogenic EGFR. Yan W, Markegard E, Dharmaiah S, Urisman A, Drew M, Esposito D, Scheffzek K, Nissley DV, McCormick F, Simanshu DK. Cell Rep. 2020 Jul 21;32(3):107909. doi: 10.1016/j.celrep.2020.107909. 10.1016/j.celrep.2020.107909 PubMed 32697994
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