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R702-X80-635: His6-tev-Hs.NF1opt (1-2818) isoform 2
(Plasmid #159575)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159575 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDest-635
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    His6-tev-Hs.NF1opt (1-2818) isoform 2
  • Species
    H. sapiens (human)
  • Entrez Gene
    NF1 (a.k.a. NFNS, VRNF, WSS)
  • Tag / Fusion Protein
    • His6-tev (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

insert contains optimized DNA sequence

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    R702-X80-635: His6-tev-Hs.NF1opt (1-2818) isoform 2 was a gift from Dominic Esposito (Addgene plasmid # 159575 ; http://n2t.net/addgene:159575 ; RRID:Addgene_159575)

  • For your References section:

    Biochemical and structural analyses reveal that the tumor suppressor neurofibromin (NF1) forms a high-affinity dimer. Sherekar M, Han SW, Ghirlando R, Messing S, Drew M, Rabara D, Waybright T, Juneja P, O'Neill H, Stanley CB, Bhowmik D, Ramanathan A, Subramaniam S, Nissley DV, Gillette W, McCormick F, Esposito D. J Biol Chem. 2020 Jan 24;295(4):1105-1119. doi: 10.1074/jbc.RA119.010934. Epub 2019 Dec 13. 10.1074/jbc.RA119.010934 PubMed 31836666
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